Трава Cirsium arvense (L.) Scop. як перспективне джерело сучасних фітопрепаратів

Abstract

Досліджено компонентний склад і кількісний вміст флавоноїдів та гідроксикоричних кислот у траві Cirsium arvense (L.) Scop. Встановлено присутність та кількісний вміст 16 флавоноїдів та 5 гідроксикоричних кислот. Кількісний вміст флавоноїдів у суцвіттях — до (3.10 ± 0.29) %, гідроксикоричних кислот — до (0.21 ± 0.02) %; вміст флавоноїдів у листі — до (2.59 ± 0.25) %, гідроксикоричних кислот — до (0.13 ± 0.01) %). Лікарська рослинна сировина C. arvense (L.) Scop. є перспективною для одержання ліофілізованих екстрактів з вираженою гепатозахисною, антиоксидантною та протизапальною дією. The aim of the work was studied the herb of Cirsium arvense (L.) Scop. of Ukrainian flora as perspective source of modern herbal drugs. The flores and leafs of C. arvense (L.) Scop. were standardizated by content of biologically active substances in flowering period. The component composition and quantitative content of flavonoids and hydroxycinnamic acids in genus herbal raw materials of C. arvense (L.) Scop. were studied. A detection оf flavonoids and hydroxycinnamic acids were conducted by chemical reactions and thin layer chromatography on «Aluminium oxide 150 F 254» (0.20 mm) (MERCK, Germany) plates. The quantitative contents of the component composition of flavonoids and hydroxycinnamic acids were conducted by HLPC chromatograph «Shimadzu LC-20 Prominence» (Japan). The chromatographic column «Phenomenex Luna C18(2)» (250 mm × 4.6 mm × 5 μm). The 16 flavonoids and 5 hydroxycinnamic acids by HPTLC were identified. Quantitative content of flavonoids in the flores (up to (3.10 ± 0.29) %), hydroxycinnamic acids (up to (0.21 ± 0.02) %); flavonoids in the leafs (up to (2.59 ± 0.25) %), hydroxycinnamic acids (up to (0.13 ± 0.01) %). Quercetin-3-O-β-D-glucopyranoside, lutelin- 5-O-β-D-glucopiranoside, gispidulin-7-O-β-D-glucopyranoside, kampherol-3-O-β-D-glucopyranoside, caftaric, protocatechuic and neochlorogenic acids firstly were identified. Prevailing of contens there were flavonoids derivates of luteolin. The method of C. arvense (L.) Scop. herbal raw materials standardization by content of the main flavonoid luteolin derivatives was proposed. The lyophilized extract of C. arvense (L.) Scop. herbal raw material is non-toxic substance. The herb of C. arvense (L.) Scop. is perspective to obtain a lyophilized extracts with hepatoprotective, antioxidant and anti-inflammatory activities.