Effect of the Spin Trapping Compound PBN and Thiotriazoline on the Outcome from Experimental Middle Cerebral Artery Occlusion in Rats

Abstract

The article aims to provide a comparative estimation of thiotriazoline’s and PBN’s neuroprotective effect on the outcome from experimental middle cerebral artery occlusion in rats. 60 Wistar rats were subjected to transient, middle cerebral artery occlusion and were randomly assigned to 3 treatment groups (n=10 each): (1) Control, (2) PBN, (3) Thiotriazoline. All rats were subjected to 90 minutes of MCA occlusion by insertion of a silicone-coated, 4-0 nylon monofilament via the external carotid artery. Investigated preparations were administered enteraly within 1 hr after operation. Functional deficits were quantified by daily neurological examinations (Garcia et al., 1995); rats’ behavior was quantified in the test of Passive Avoidance Conditioned Response (PACR). Infarct volume was assessed after 7 days. Nitrotyrosine value was measured after 3 days. PBN and thiotriazoline reduced total infarct volume by 36.8%, and 24.7%, respectively, relative to controls. PBN and thiotriazoline therapy reduced infarct volume in THE basal ganglia. Compared with vehicle-treated controls, the infarct volume in the basal ganglia in ats treated with PBN was 24.2±6.2 mm3 and in rats treated with thiotriazoline, it was 18.2±4.8 mm3.PBN and thiotriazoline, caused major reduction of ischemic brain damage when administered in a single dose 60 min after onset of MCAO. This benefit was evident both histologically and neurologically in rats allowed to survive 7 d after the ischemic insult. Animals treated with thiotriazoline had the best neurological recovery, the fewest postoperative cognitive deficits, and the smallest infarct volume.