Nitrozine Stress and Neurological Disorders in Experimental Alcohol Intoxication and Their Pharmacological Correction by Neuropeptides

Abstract

The article aims to provide a comparative estimation of Cerebrocurin®, Cortexin® and Cerebrolisin® neuroprotective effect on the outcome of experimental chronic alcoholism in rats. 50 Wistar rats were subjected to transient, experimental chronic alcoholism and were randomly assigned to 5 groups (n=10 each): (1) Intact, (2) Control, (3) Cerebricurin®, (4) Cortexin®, (5) Cerebrolisin®. Investigated preparations were administered during 14 days parenteraly after 30-days of violent alcoholization. Functional deficits were quantified by daily neurological examinations (Garcia et al., 1995); rats’ behavior was quantified in the test of Passive Avoidance Conditioned Response (PACR). Nitrotirosine values were measured after treatment. Against the background of chronic alcohol intoxication in rats, we have elevated indicators, nitrozile proteins in plasma and brain reflecting the activation processes of nitrozine stress in each groups. We have conducted correlation between the level of nitrotirozine in rat brain and the manifestations of neurological deficit in scores on McGrow in the control group at the end of the experiment. The most active drug was Cerebrocurin®, which demonstrated a significant reduction of nitrotirozine in plasma and especially in the brain of the rats relative vs vehicle-treated controls and normalized neurological status. This is an experimental justification for inclusion of Cerebrocurin® in the traditional model of treatment of chronic alcoholism.