Пошук шляхів корекції дисфункції тимуса у щурів з експериментальним цукровим діабетом

Abstract

We investigated the influence of multiple introductions of NO precursor L-arginine and NOS non-selective blocker N-nitro- L-arginine (NNLA) on thymic morpho-functional status in Wistar male rats with experimental diabetes mellitus (EDM). To reveal insulin-expressing, proliferating, Treg-cells, iNOS+- cells and Bcl-2+-cells, the immunohistochemical methods of direct and indirect immunofluorescence with monoclonal antibodies to insulin, PCNA, CD-25 antigen. Bcl-2 and iNOS of rat were used. It was established that NNLA administration to rats with EDM has more pronounced effect in comparison with L-arginine administration, demonstrating an increase in the number of Treg-cells, insulin-expressing and proliferating thymocytes and a decrease in the density of iNOS+- and Bcl- 2+-cells population.