Содержания флавоноидов и гидроксикоричных кислот в растительном сырье Plantago media L. и Plantago altissima L. флоры Украины

Abstract

Plant raw material of species Plantago (L.) family Plantaginaceae is known as a promising source of biologically active substances: flavonoids, hydroxycinnamic acids, polysaccharides, vitamin K1, C, terpenoids, aucubin, etc. The aim of our research was study the composition of flavonoids and hydroxycinnamic acids in the leaves of Plantago media L. and Plantago altissima L. of the flora of Ukraine. Qualitative reactions and thin layer chromatography on Sorbfil AF-A plates were used to identify the compounds. The content of individual compounds of flavonoids and hydroxycinnamic acids was determined by HPLC. The presence of chlorogenic and non-chlorogenic acids and luteolin was reliably detected in the researched samples in result of comparison with standard samples. By the means of Rf, the presence of acteoside and plantamoyoside was established. In HPLC analyzes, it was determined that the leaves of Plantago media L. and Plantago altissima L. accumulated identical biologically active flavonoids and hydroxycinnamic acids during flowering. In both species are presence 5 substances from the class of flavonoids (luteolin-7-O-β-D-glucopyranoside, luteolin, kaempferol, apigenin, apigenin-7-O-β-D-glucopyranoside) and up to 11 substances from the class of hydroxycinnamic acids. The results of our research show that plant raw materials (leaves) of Plantago media L. and Plantago altissima L. accumulate high concentrations of flavonoids and hydroxycinnamic acids and can be recommended to do of phytopreparations.