Neuroprotective effect of selective estrogen receptor modulator (serm) in deprivation of the system`s level of restored glutathione in vitro: HSP70 role in the implementation of this action

Abstract

The aim of the research: to determine the value of the heat shock protein HSP 70 in the implementation of the mechanism of neuroprotective actions of selective estrogen receptor modulator (SERM) – (Z)-2-[4-(1,2-diphenyl-1-butenil)fenoxy]-N,N-dimethilaethanamin citrate. The objective of this study was to estimate the influence of SERM on the expression of HSP70, the activity of the thiol-disulfide system of neurons and mitochondria development of neuronal apoptosis in vitro, with a deficit of restored glutathione. Materials and Methods: neurons of cortex isolated extemporale from the brain of a week-long white outbred rats. Deficit of glutathione caused by the introduction into suspension of neurons of D,L-butionin-S.R-sulfoxime (BSO,500 мкМ). The agent was selected on ability to influence the intracellular synthesis of glutathione. Apoptotic modified neurons identified by painting of the etodium bromide, the expression of HSP 70 was determined by method of immunoblotting. In the mitochondria and citosole determined the content of restored glutathione, markers of oxidative modification of proteins. Also determined the charge of the mitochondrial membrane and level of opening of the mitochondrial permeability transition pore.