Pecularirties of innate immunity receptors expression by lymphocytes of mesenteric lymph nodes in offspring of experimental gestational diabetes rat

Abstract

The purpose of the research was to find out the distribution of TLR2+-, TLR4+-, NOD2+- and RIGI+- lymphocytes in mesenteric lymph nodes in the offspring of experimental gestational diabetes (EGD) rats, in the offspring of rats with EGD whose mothers received glibenclamide and the descendants of rats from EGD who received oral insulin for 14 days after birth. Materials and methods. For the identification of PRR+-lymphocytes was used an indirect immunofluorescence method using monoclonal or polyclonal antibodies. Results. Experimental gestational diabetes leads to an increase in the number of TLR2+, TLR4+, NOD2+ and RIGI+ lymphocytes in MLN in the offspring and changes the density of PRRs on immune cells. Changes are more pronouncedly in age 1 month of life. In conditions of formation of oral tolerance to insulin, the number of cortex TLR2+ and TLR4+ lymphocytes in 1-month offspring decreases. The amount of TLR2+ and RIG-I+ cells in medullary cords of these rats decreases too. The dynamics in the number of immunoreactive cells to PRRs in the cortex of the MLN remains up to 6 months of age. Administration of glibenclamide to pregnant females reduces the number of TLR4+ and RIG-I+ lymphocytes in the MLN of 1-month offspring, but only TLR2+ cells in 6-month-old rats. It does not affect their number in medullary cords and decrease preferentially the PRRs density on immunopositive MLN lymphocytes in early terms of observation. Conclusions. The introduction of insulin to offspring and glibenclamide to pregnant females reduces the level of activation of the innate immunity receptors in the MLN in offspring.