Spectrophotometric determination of metoprolol tartrate in pure and dosage forms

Abstract

Objective: A new spectrophotometric method has been developed for the determination of metoprolol tartrate in pure and dosage forms. Material and Method: This method is based on the reaction between metoprolol tartrate and 2,3-dichloro-1,4-naphthoquinone in dimethylformamide (DMF) medium to form the colored reaction product with maximum absorption at 493 nm. Optimum conditions to carry out the reaction such as concentration of reagent, temperature and heating time were carefully studied and optimized. Beer’s law was performed at the concentration range of 18.00-28.00 mg/100 ml. The proposed method is valid according to the validation requirements of the State Pharmacopoeia of Ukraine. Result and Discussion: The results of the study show that the procedure is accurate, simple and relevant for application at the quality control laboratories for dosage forms.